Augment data

This function takes a seurat object and finds cells that are not sufficiently abundant when grouped by the column parameter, then simulates data to augment cell number to a level of the parameter - norm_number

Usage

augment_data(obj, column, norm_number = 2000, assay = "RNA", prune = F)

Arguments

column

column from the metadata that designates cell group (i.e. celltype)

norm_number

cell number to augment data to for cells that are not sufficiently abundant in the object

prune

downsample cells present at number higher than norm_number to the level of norm_number (default = F)

Value

a seurat object augmented with simulated cells such that all cell groups are present at a level of norm_number of cells